A plasmid is a circle Of DNA with lots of parts Bacteria make ‘em But scientists can break ‘em apart So now I’m so glad That we can design ‘em a la carte So they should be shared, be shared, be shared Plasmids turn genetics into art Thinking about playing with genes What if we could change 'em, and spread 'em like memes? What if we could rearrange 'em, to study proteins? Well these plasmids got us all letting of S.T.E.A.M. It's lit. So here's what a plasmid is Circle of DNA from bacterias It's got restriction sites where we slash with scissors called restriction enzymes that let us match and mix Or mix and match genes from new species. Just put in a promoter And express with ease And with a site of origin it can replicate To make copies of your plasmid and generate Yeah they're great – easy to manipulate But just think, what if they could infiltrate Not just bacteria but other life? We'd be pretty much wielding a genetic knife A plasmid is a circle Of DNA with lots of parts Bacteria make ‘em But scientists can break ‘em apart So now I’m so glad That we can design ‘em a la carte So they should be shared, be shared, be shared Plasmids turn genetics into art OK Snip. Poke. Test cells Pipette Run gels Ligate Incubate Die? Wait. Survive? Great. Now each ring has a power like Frodo done told me (Baggins) And I’ve got so many you can call me Kobe (Swaggin) Favorite application is to make 'em glowing Which helps you find out where molecules are going All I do is insert GFP In the right type of ring with right set of genes Depending how I do it it'll be glowing green Or blue – either all the time or when I want it to Like maybe just in cells of a certain type Or biosensor for molecules floating by And I can do this in fish, I can do this in a beagle I could do it to you (but that'd be illegal). But we do it to research the state of nature Golden age of biotech and its applications From medicine to food to security To learning about the fundamentals of biology Plasmids What new genes can we add in? What new questions we asking? Plasmids Plasmids Plasmids What new genes can we add in? What new questions we asking? Plasmids Plasmids Hey everybody I'm Tom Thank you so much for watching and huge thanks to Addgene – the nonprofit plasmid repository for sponsoring the video as well as the Doudna RNA lab at Berkeley. If your organization wants a music video or another type of video about whatever it is you do, talk to me. And don't forget to subscribe to Science With Tom. We've got a ton in the works right now. See you soon!